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FEMS Microbiol Lett. 2014 Feb;351(2):147-55. doi: 10.1111/1574-6968.12332. Epub 2013 Dec 16.

Discovery of two novel β-glucosidases from an Amazon soil metagenomic library.

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1
Genomic Sciences and Biotechnology Program, Universidade Católica de Brasília, Brasília, Brazil.

Abstract

An Amazon soil microbial community metagenomic fosmid library was functionally screened for β-glucosidase activity. Contig analysis of positive clones revealed the presence of two ORFs encoding novel β-glucosidases, AmBGL17 and AmBGL18, from the GH3 and GH1 families, respectively. Both AmBGL17 and AmBGL18 were functionally identified as β-glucosidases. The enzymatic activity of AmBGL17 was further characterized. AmBGL17 was tested with different substrates and showed highest activity on pNPβG substrate with an optimum temperature of 45 °C and an optimum pH of 6. AmBGL17 showed a Vmax of 116 mM s(-1) and Km of 0.30 ± 0.017 mM. This is the first report of β-glucosidases from an Amazon soil microbial community using a metagenomic approach.

KEYWORDS:

GH1; GH3; beta-glucosidase; functional screen; metagenomic library

PMID:
24236615
DOI:
10.1111/1574-6968.12332
[Indexed for MEDLINE]
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