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Nucleic Acids Res. 2014 Jan;42(Database issue):D780-8. doi: 10.1093/nar/gkt1092. Epub 2013 Nov 13.

FlyBase 102--advanced approaches to interrogating FlyBase.

Author information

1
The Biological Laboratories, Harvard University, 16 Divinity Avenue, Cambridge, MA 02138, USA and Department of Genetics, University of Cambridge, Downing Street, Cambridge CB2 3EH, UK.

Abstract

FlyBase (http://flybase.org) is the leading website and database of Drosophila genes and genomes. Whether you are using the fruit fly Drosophila melanogaster as an experimental system or wish to understand Drosophila biological knowledge in relation to human disease or to other model systems, FlyBase can help you successfully find the information you are looking for. Here, we demonstrate some of our more advanced searching systems and highlight some of our new tools for searching the wealth of data on FlyBase. The first section explores gene function in FlyBase, using our TermLink tool to search with Controlled Vocabulary terms and our new RNA-Seq Search tool to search gene expression. The second section of this article describes a few ways to search genomic data in FlyBase, using our BLAST server and the new implementation of GBrowse 2, as well as our new FeatureMapper tool. Finally, we move on to discuss our most powerful search tool, QueryBuilder, before describing pre-computed cuts of the data and how to query the database programmatically.

PMID:
24234449
PMCID:
PMC3964969
DOI:
10.1093/nar/gkt1092
[Indexed for MEDLINE]
Free PMC Article

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