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Methods Mol Biol. 2014;1101:267-303. doi: 10.1007/978-1-62703-721-1_13.

Construction and application of site-specific artificial nucleases for targeted gene editing.

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  • 1Program in Gene Function & Expression, Department of Biochemistry & Molecular Pharmacology, UMass Medical School, Worcester, MA, USA.

Abstract

Artificial nucleases have developed into powerful tools for introducing precise genome modifications in a wide variety of species. In this chapter the authors provide detailed protocols for rapidly constructing zinc finger nucleases (ZFNs) and TALE nucleases (TALENs) and evaluating their activity for the targeted generation of InDels within the zebrafish genome.

PMID:
24233786
DOI:
10.1007/978-1-62703-721-1_13
[PubMed - indexed for MEDLINE]
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