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Dev Cell. 2013 Nov 11;27(3):345-52. doi: 10.1016/j.devcel.2013.10.005.

Cytoplasmic streaming velocity as a plant size determinant.

Author information

1
Live Cell Molecular Imaging Research Team, RIKEN Center for Advanced Photonics, Wako, Saitama 351-0198, Japan; Japan Science and Technology Agency, PRESTO, 4-1-8 Honcho Kawaguchi, Saitama 332-0012, Japan. Electronic address: tominaga@riken.jp.

Abstract

Cytoplasmic streaming is active transport widely occurring in plant cells ranging from algae to angiosperms. Although it has been revealed that cytoplasmic streaming is generated by organelle-associated myosin XI moving along actin bundles, the fundamental function in plants remains unclear. We generated high- and low-speed chimeric myosin XI by replacing the motor domains of Arabidopsis thaliana myosin XI-2 with those of Chara corallina myosin XI and Homo sapiens myosin Vb, respectively. Surprisingly, the plant sizes of the transgenic Arabidopsis expressing high- and low-speed chimeric myosin XI-2 were larger and smaller, respectively, than that of the wild-type plant. This size change correlated with acceleration and deceleration, respectively, of cytoplasmic streaming. Our results strongly suggest that cytoplasmic streaming is a key determinant of plant size. Furthermore, because cytoplasmic streaming is a common system for intracellular transport in plants, our system could have applications in artificial size control in plants.

PMID:
24229646
DOI:
10.1016/j.devcel.2013.10.005
[Indexed for MEDLINE]
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