Pretreatment of urine samples with SDS improves direct identification of urinary tract pathogens with matrix-assisted laser desorption ionization-time of flight mass spectrometry

F Sánchez-Juanes; M Siller Ruiz; F Moreno Obregón; M Criado González; S Hernández Egido; M de Frutos Serna; J M González-Buitrago; J L Muñoz-Bellido

doi:10.1128/JCM.01881-13

Pretreatment of urine samples with SDS improves direct identification of urinary tract pathogens with matrix-assisted laser desorption ionization-time of flight mass spectrometry

J Clin Microbiol. 2014 Jan;52(1):335-8. doi: 10.1128/JCM.01881-13. Epub 2013 Nov 13.

Authors

F Sánchez-Juanes¹, M Siller Ruiz, F Moreno Obregón, M Criado González, S Hernández Egido, M de Frutos Serna, J M González-Buitrago, J L Muñoz-Bellido

Affiliation

¹ Unidad de Investigación, Complejo Asistencial Universitario de Salamanca, Salamanca, Spain.

Abstract

We pretreated with SDS 71 urine samples with bacterial counts of >10(5) CFU/ml and matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) identification scores of <2, in order to minimize failure rates. Identification improved in 46.5% of samples, remained unchanged in 49.3%, and worsened in 4.2%. The improvement was more evident for Gram-negative (54.3%) than for Gram-positive (32%) bacteria.

MeSH terms

Bacteria / classification
Bacteria / isolation & purification*
Bacterial Infections / diagnosis*
Detergents / pharmacology
Humans
Microbiological Techniques / methods*
Sodium Dodecyl Sulfate / pharmacology
Specimen Handling / methods*
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
Urinary Tract Infections / diagnosis*
Urine / microbiology*

Substances

Detergents
Sodium Dodecyl Sulfate