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Methods Cell Biol. 1986;27:309-23.

Embryo dissociation, cell isolation, and cell reassociation.

Abstract

In resolving the role of cell recognition events in the process of morphogenesis it is necessary to focus on single events against a background of many complex interactions. This article presents a series of approaches that are designed to do just that. It should be noted that with simplification there is a danger of oversight. Nevertheless, in learning about mechanisms of cellular movement at gastrulation we have found that cell separation techniques, simplified adhesion assays, and predictable antibody activities are helpful for approaching the complex mechanisms of morphogenesis.

PMID:
2422529
DOI:
10.1016/s0091-679x(08)60355-6
[Indexed for MEDLINE]

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