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PLoS One. 2013 Nov 4;8(11):e73798. doi: 10.1371/journal.pone.0073798. eCollection 2013.

Urinary exosomal microRNAs in incipient diabetic nephropathy.

Author information

1
Diabetic Nephropathy Laboratory, Department of Medical Science, University of Turin, Turin, Italy.

Abstract

MicroRNAs (miRNAs), a class of small non-protein-encoding RNAs, regulate gene expression via suppression of target mRNAs. MiRNAs are present in body fluids in a remarkable stable form as packaged in microvesicles of endocytic origin, named exosomes. In the present study, we have assessed miRNA expression in urinary exosomes from type 1 diabetic patients with and without incipient diabetic nephropathy. Results showed that miR-130a and miR-145 were enriched, while miR-155 and miR-424 reduced in urinary exosomes from patients with microalbuminuria. Similarly, in an animal model of early experimental diabetic nephropathy, urinary exosomal miR-145 levels were increased and this was paralleled by miR-145 overexpression within the glomeruli. Exposure of cultured mesangial cells to high glucose increased miR-145 content in both mesangial cells and mesangial cells-derived exosomes, providing a potential mechanism for diabetes-induced miR-145 overexpression. In conclusion, urinary exosomal miRNA content is altered in type 1 diabetic patients with incipient diabetic nephropathy and miR-145 may represent a novel candidate biomarker/player in the complication.

PMID:
24223694
PMCID:
PMC3817183
DOI:
10.1371/journal.pone.0073798
[Indexed for MEDLINE]
Free PMC Article

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