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Anticancer Res. 2013 Nov;33(11):5199-203.

Aberrant methylation of the Ras-related associated with diabetes gene in human primary esophageal cancer.

Author information

1
The Shenzhen University School of Medicine. 3688 Nanhai Ave, Rm 703, Nanshan, Shenzhen, Guangdong, People's Republic of China 518060. Tel: +86 075586671904, zhejin1995@yahoo.com.

Abstract

BACKGROUND/AIM:

Ras-related associated with diabetes (RRAD), a member of the Ras-related GTPase superfamily, is frequently methylated in several human cancers, though its methylation profile remains unclear in esophageal cancer.

MATERIALS AND METHODS:

We examined RRAD promoter hypermethylation using real-time quantitative methylation-specific PCR in 229 primary human esophageal tissues of contrasting histological types.

RESULTS:

RRAD hypermethylation showed highly discriminative receiver-operator characteristic curve profiles, clearly distinguishing esophageal squamous cell carcinoma (ESCC) from esophageal adenocarcinoma (EAC) or normal esophagus (NE) (p<0.01 and p<0.01, respectively). RRAD normalized methylation values were significantly higher in ESCC (0.0242) than in NE (0.0057, p<0.05) or EAC (0.0139, p<0.01). RRAD hypermethylation frequency was also significantly higher in ESCC (23.1%) than in NE (0%, p<0.05) or EAC (5.4%, p<0.05).

CONCLUSION:

Promoter hypermethylation of RRAD is a frequent, tissue-specific event in ESCC, and is uncommon in EAC. The aberrant methylation of RRAD may be involved in the pathogenesis of a subset of ESCC, but not in EAC.

KEYWORDS:

EAC; ESCC; RRAD; hypermethylation

PMID:
24222170
[Indexed for MEDLINE]
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