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Biochem Biophys Res Commun. 2013 Nov 29;441(4):976-81. doi: 10.1016/j.bbrc.2013.11.006. Epub 2013 Nov 9.

MicroRNA-490-5p inhibits proliferation of bladder cancer by targeting c-Fos.

Author information

1
Department of Urology, The First Affiliated Hospital, School of Medicine, Zhejiang University, Qingchun Road 79, Hangzhou 310003, Zhejiang, PR China.

Abstract

MicroRNAs (miRNAs) are non-protein-coding sequences that play a crucial role in tumorigenesis by negatively regulating gene expression. Here, we found that miR-490-5p is down-regulated in human bladder cancer tissue and cell lines compared to normal adjacent tissue and a non-malignant cell line. To better characterize the function of miR-490-5p in bladder cancer, we over-expressed miR-490-5p in bladder cancer cell lines with chemically synthesized mimics. Enforced expression of miR-490-5p in bladder cancer cells significantly inhibited the cell proliferation via G1-phase arrest. Further studies found the decreased c-Fos expression at both mRNA and protein levels and Luciferase reporter assays demonstrated that c-Fos is a direct target of miR-490-5p in bladder cancer. These findings indicate miR-490-5p to be a novel tumor suppressor of bladder cancer cell proliferation through targeting c-Fos.

KEYWORDS:

Bladder cancer; Proliferation; c-Fos; miR-490-5p

PMID:
24220339
DOI:
10.1016/j.bbrc.2013.11.006
[Indexed for MEDLINE]
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