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Zhonghua Nan Ke Xue. 2013 Oct;19(10):902-6.

[Bulky DNA adducts in human sperm correlate with semen parameters and sperm apoptosis].

[Article in Chinese]

Author information

1
Nanjing Research Institute of Environmental Science, Nanjing, Jiangsu 210042, China. jgx@nies.org

Abstract

OBJECTIVE:

To explore the correlation of sperm polycyclic aromatic hydrocarbons (PAH)-DNA adducts with semen quality and sperm apoptosis.

METHODS:

We collected semen samples from 433 infertile Chinese men, detected sperm PAH-DNA adducts using immunofluorescence staining and flow cytometry, and determined the rate of sperm apoptosis by TUNEL.

RESULTS:

Multiple linear regression analysis showed that the level of sperm PAH-DNA adducts was correlated negatively with sperm concentration (beta = -0.632), total sperm count (beta = -0.830) and sperm motility (beta = -9.647), but positively with the rate of sperm apoptosis (beta = 0.130).

CONCLUSION:

Sperm PAH-DNA adducts are evidently correlated with semen quality and sperm apoptosis, and play an important role in the evaluation of male productivity.

PMID:
24218944
[Indexed for MEDLINE]

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