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Methods Mol Biol. 2014;1100:169-76. doi: 10.1007/978-1-62703-724-2_14.

Purification and functional characterization of C4b-binding protein (C4BP).

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  • 1Department of Laboratory Medicine, Section of Medical Protein Chemistry, The Wallenberg Laboratory, Lund University, Skåne University Hospital, Malmö, Sweden.


C4b-binding protein (C4BP) is a soluble, 570 kDa large glycoprotein, present in plasma at a concentration of approximately 200 mg/L. C4BP is the main inhibitor of the classical and lectin pathways of complement, where it controls C4b-mediated reactions. Here, we describe a method for purification of C4BP from human plasma, which is based on barium chloride precipitation, anion exchange chromatography, and gel filtration. We also describe a functional assay, in which C4BP's cofactor activity to factor I, in the degradation of C4b, can be assessed.

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