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Metab Eng. 2014 Jan;21:17-25. doi: 10.1016/j.ymben.2013.10.012. Epub 2013 Nov 9.

Metabolic engineering of Thermoanaerobacterium saccharolyticum for n-butanol production.

Author information

1
Thayer School of Engineering, 14 Engineering Drive, Hanover, NH 03755, USA.
2
Mascoma Corporation, 67 Etna Rd, Lebanon, NH 03766, USA.
3
Universität Rostock, Albert-Einstein-Str. 3, Rostock 18051, Germany.
4
Thayer School of Engineering, 14 Engineering Drive, Hanover, NH 03755, USA; Mascoma Corporation, 67 Etna Rd, Lebanon, NH 03766, USA. Electronic address: lee.r.lynd@dartmouth.edu.

Abstract

The thermophilic anaerobe Thermoanaerobacterium saccharolyticum JW/SL-YS485 was investigated as a host for n-butanol production. A systematic approach was taken to demonstrate functionality of heterologous components of the clostridial n-butanol pathway via gene expression and enzymatic activity assays in this organism. Subsequently, integration of the entire pathway in the wild-type strain resulted in n-butanol production of 0.85 g/L from 10 g/L xylose, corresponding to 21% of the theoretical maximum yield. We were unable to integrate the n-butanol pathway in strains lacking the ability to produce acetate, despite the theoretical overall redox neutrality of n-butanol formation. However, integration of the n-butanol pathway in lactate deficient strains resulted in n-butanol production of 1.05 g/L from 10 g/L xylose, corresponding to 26% of the theoretical maximum.

KEYWORDS:

Biofuels; Metabolic engineering; Thermophiles; n-Butanol

PMID:
24216277
DOI:
10.1016/j.ymben.2013.10.012
[Indexed for MEDLINE]

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