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Anal Biochem. 1986 Feb 1;152(2):304-7.

Acceleration of nucleic acid hybridization rate by polyethylene glycol.

Abstract

The addition of polyethylene glycol to filter-bound nucleic acid hybridization greatly increases the hybridization rate. With single-stranded probes, the increase obtained with polyethylene glycol is significantly greater than that obtained with dextran sulfate. Additionally, polyethylene glycol is easier to manipulate and less expensive than dextran sulfate.

PMID:
2421601
DOI:
10.1016/0003-2697(86)90413-6
[Indexed for MEDLINE]

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