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Clinics (Sao Paulo). 2013 Oct;68(10):1338-43. doi: 10.6061/clinics/2013(10)08.

Tocotrienol supplementation in postmenopausal osteoporosis: evidence from a laboratory study.

Author information

1
Universiti Kebangsaan, UKM Medical Faculty Jalan Raja Muda Abdul Aziz, Pharmacology Department, Kuala Lumpur, Malaysia.

Abstract

OBJECTIVE:

Accelerated bone loss that occurs in postmenopausal women has been linked to oxidative stress and increased free radicals. We propose the use of antioxidants to prevent and reverse postmenopausal osteoporosis. This study aimed to examine the effects of tocotrienol, a vitamin E analog, on bone loss due to estrogen deficiency. Our previous study showed that tocotrienol increased the trabecular bone volume and trabecular number in ovariectomized rats. In the current study, we investigated the effects of tocotrienol supplementation on various biochemical parameters in a postmenopausal osteoporosis rat model.

MATERIALS AND METHODS:

A total of 32 female Wistar rats were randomly divided into four groups. The baseline group was sacrificed at the start of the study, and another group was sham operated. The remaining rats were ovariectomized and either given olive oil as a vehicle or treated with tocotrienol at a dose of 60 mg/kg body weight. After four weeks of treatment, blood was withdrawn for the measurement of interleukin-1 (IL1) and interleukin-6 (IL6) (bone resorbing cytokines), serum osteocalcin (a bone formation marker) and pyridinoline (a bone resorption marker).

RESULTS:

Tocotrienol supplementation in ovariectomized rats significantly reduced the levels of osteocalcin, IL1 and IL6. However, it did not alter the serum pyridinoline level.

CONCLUSION:

Tocotrienol prevented osteoporotic bone loss by reducing the high bone turnover rate associated with estrogen deficiency. Therefore, tocotrienol has the potential to be used as an anti-osteoporotic agent in postmenopausal women.

PMID:
24212841
PMCID:
PMC3798611
DOI:
10.6061/clinics/2013(10)08
[Indexed for MEDLINE]
Free PMC Article

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