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Biochem Biophys Res Commun. 2013 Dec 6;442(1-2):16-21. doi: 10.1016/j.bbrc.2013.10.126. Epub 2013 Nov 6.

Proteomic analysis of Girdin-interacting proteins in migrating new neurons in the postnatal mouse brain.

Author information

1
Department of Developmental and Regenerative Biology, Nagoya City University, Graduate School of Medical Sciences, Aichi, Japan; Department of Anesthesiology and Medical Crisis Management, Nagoya City University, Graduate School of Medical Sciences, Aichi, Japan.

Abstract

Neural stem cells continuously generate new neurons in the ventricular-subventricular zone (V-SVZ) of the postnatal and adult mammalian brain. New neurons born in the rodent V-SVZ migrate toward the olfactory bulb (OB), where they differentiate into interneurons. To reveal novel intracellular molecular mechanisms that control postnatal neuronal migration, we performed a global proteomic search for proteins interacting with Girdin, an essential protein for postnatal neuronal migration. Using GST pull-down and LC-MS/MS shotgun analysis, we identified cytoskeletal proteins, cytoskeleton-binding proteins, and signal-transduction proteins as possible participants in neuronal migration. Our results suggest that Girdin and Girdin-interacting proteins control neuronal migration by regulating actin and/or microtubule dynamics.

KEYWORDS:

Girdin; Liquid chromatography tandem mass spectrometry (LC–MS/MS); Neuronal migration; Postnatal neurogenesis; Ventricular–subventricular zone (V–SVZ)

PMID:
24211587
DOI:
10.1016/j.bbrc.2013.10.126
[Indexed for MEDLINE]

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