Send to

Choose Destination
Cell Rep. 2013 Nov 27;5(4):1082-94. doi: 10.1016/j.celrep.2013.10.013. Epub 2013 Nov 7.

High-frequency promoter firing links THO complex function to heavy chromatin formation.

Author information

Centre de Génétique Moléculaire, CNRS-UPR 3404, 91190 Gif sur Yvette, France; Centre for mRNP Biogenesis and Metabolism, Aarhus University, 8000 Aarhus, Denmark.


The THO complex is involved in transcription, genome stability, and messenger ribonucleoprotein (mRNP) formation, but its precise molecular function remains enigmatic. Under heat shock conditions, THO mutants accumulate large protein-DNA complexes that alter the chromatin density of target genes (heavy chromatin), defining a specific biochemical facet of THO function and a powerful tool of analysis. Here, we show that heavy chromatin distribution is dictated by gene boundaries and that the gene promoter is necessary and sufficient to convey THO sensitivity in these conditions. Single-molecule fluorescence in situ hybridization measurements show that heavy chromatin formation correlates with an unusually high firing pace of the promoter with more than 20 transcription events per minute. Heavy chromatin formation closely follows the modulation of promoter firing and strongly correlates with polymerase occupancy genome wide. We propose that the THO complex is required for tuning the dynamic of gene-nuclear pore association and mRNP release to the same high pace of transcription initiation.

[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center