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J Pediatr. 2014 Jan;164(1):189-191.e1. doi: 10.1016/j.jpeds.2013.09.025. Epub 2013 Oct 25.

Comparison of frozen and unfrozen blood spots for gene expression studies.

Author information

1
Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI.
2
Laboratory of Microarray Technology, Van Andel Research Institute, Grand Rapids, MI.
3
Laboratory of Genital Tract Biology, Department of Obstetrics, Gynecology, and Reproductive Biology, Harvard Medical School and Brigham and Women's Hospital, Boston, MA.
4
Neuroepidemiology Unit, Department of Neurology, Boston Children's Hospital, Harvard University, Boston, MA.
5
Department of Epidemiology and Biostatistics, College of Human Medicine, Michigan State University, East Lansing, MI; Department of Pediatrics and Human Development, College of Human Medicine, Michigan State University, East Lansing, MI. Electronic address: paneth@msu.edu.

Abstract

We studied gene expression in 9 sets of paired newborn blood spots stored for 8-10 years in either the frozen state or the unfrozen state. Fewer genes were expressed in unfrozen spots, but the average correlation coefficient for overall gene expression comparing the frozen and unfrozen state was 0.771 (95% CI, 0.700-0.828).

KEYWORDS:

ELGAN; Extremely Low Gestational Age Newborns

PMID:
24209717
PMCID:
PMC4326080
DOI:
10.1016/j.jpeds.2013.09.025
[Indexed for MEDLINE]
Free PMC Article

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