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ACS Appl Mater Interfaces. 2013 Nov 27;5(22):11520-4. doi: 10.1021/am4040448. Epub 2013 Nov 13.

Enhanced PCR amplification of GC-rich DNA templates by gold nanoparticles.

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1
School of Biomedical Engineering and ‡Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University , Shanghai 200240, China.

Abstract

Gold nanoparticles (AuNPs) have been reported to facilitate double-stranded DNA dissociation and improve performance of several PCR systems. Here we investigated AuNPs' effect on GC-rich DNA amplification. We found that AuNPs could enhance PCR amplification of the GNAS1 promoter region (∼84% GC) mediated by Pfu or Taq DNA polymerase. However, under optimal concentrations of AuNPs, higher amounts of Taq were required. Furthermore, the GC-rich FMR1 (80.4% GC) gene of Homo sapiens as well as exoT (67.3% GC), exsE (71% GC) and pqqF genes (74% GC) of Pseudomonas aeruginosa were also efficiently amplified. AuNPs can become an effective additive in GC-rich PCR and facilitate analysis of challenging genomic sequence in basic and clinical research.

PMID:
24206448
DOI:
10.1021/am4040448
[Indexed for MEDLINE]
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