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J Biol Chem. 2013 Dec 20;288(51):36484-91. doi: 10.1074/jbc.M113.472324. Epub 2013 Nov 5.

The protein deacetylase SIRT3 prevents oxidative stress-induced keratinocyte differentiation.

Author information

1
From the Department of Cell Biology, Harvard Medical School, Boston, Massachusetts 02115 and.

Abstract

Keratinocyte differentiation is a key process in the formation and maintenance of the protective skin barrier. Dysregulation in the balance of reactive oxygen species homeostasis may play a role in keratinocyte differentiation. We have identified the mitochondrial deacetylase SIRT3 as a key regulator of mitochondrial reactive oxygen species in keratinocytes. Our studies demonstrate that SIRT3 expression is down-regulated during keratinocyte differentiation, consistent with an increase in mitochondrial superoxide levels. Importantly, loss of SIRT3 expression in keratinocytes increased superoxide levels and promoted the expression of differentiation markers, whereas overexpression decreased superoxide levels and reduced the expression of differentiation markers. These findings identify a new role for SIRT3 in the suppression of epidermal differentiation via lowering oxidative stress.

KEYWORDS:

Differentiation; Keratinocytes; Mitochondria; Reactive Oxygen Species (ROS); SIRT3; Sirtuins

PMID:
24194516
PMCID:
PMC3868761
DOI:
10.1074/jbc.M113.472324
[Indexed for MEDLINE]
Free PMC Article

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