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Genetics. 2013 Nov;195(3):635-42. doi: 10.1534/genetics.113.156521.

Exciting prospects for precise engineering of Caenorhabditis elegans genomes with CRISPR/Cas9.

Author information

1
Howard Hughes Medical Institute, Department of Biology, University of Utah, Salt Lake City, Utah 84112 and Department of Biomedical Sciences and Danish National Research Foundation Centre for Cardiac Arrhythmia, University of Copenhagen, 2200 Kbh N, Denmark.

Abstract

With remarkable speed, the CRISPR-Cas9 nuclease has become the genome-editing tool of choice for essentially all genetically tractable organisms. Targeting specific DNA sequences is conceptually simple because the Cas9 nuclease can be guided by a single, short RNA (sgRNA) to introduce double-strand DNA breaks (DSBs) at precise locations. Here I contrast and highlight protocols recently developed by eight different research groups, six of which are published in GENETICS, to modify the Caenorhabditis elegans genome using CRISPR/Cas9. This reverse engineering tool levels the playing field for experimental geneticists.

PMID:
24190921
PMCID:
PMC3813854
DOI:
10.1534/genetics.113.156521
[Indexed for MEDLINE]
Free PMC Article

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