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Nat Med. 2013 Dec;19(12):1655-60. doi: 10.1038/nm.3384. Epub 2013 Nov 3.

Common noncoding UMOD gene variants induce salt-sensitive hypertension and kidney damage by increasing uromodulin expression.

Author information

1
Dulbecco Telethon Institute, c/o Division of Genetics and Cell Biology, San Raffaele Scientific Institute, Milan, Italy.
2
Institute of Physiology, Zurich Centre for Integrative Human Physiology, University of Zurich, Zurich, Switzerland.
3
Division of Nephrology, UCL Medical School, Brussels, Belgium.
4
Division of Nephrology and Dialysis, San Raffaele Scientific Institute, Milan, Italy.
5
Renal Research Laboratory, Fondazione IRCCS Ospedale Maggiore Policlinico & Fondazione D'Amico per la Ricerca sulle Malattie Renali, Milan, Italy.
6
INSERM, UMRS 872, Paris, France.
7
University Vita-Salute San Raffaele, Milan, Italy.
8
Department of Cardiovascular Research, Istituto di Ricerca Farmacologica Mario Negri, Milan, Italy.
9
Department of Pathology, San Raffaele Scientific Institute, Milan, Italy.
10
Institute of Anatomy, Zurich Centre for Integrative Human Physiology, University of Zurich, Zurich, Switzerland.
#
Contributed equally

Abstract

Hypertension and chronic kidney disease (CKD) are complex traits representing major global health problems. Multiple genome-wide association studies have identified common variants in the promoter of the UMOD gene, which encodes uromodulin, the major protein secreted in normal urine, that cause independent susceptibility to CKD and hypertension. Despite compelling genetic evidence for the association between UMOD risk variants and disease susceptibility in the general population, the underlying biological mechanism is not understood. Here, we demonstrate that UMOD risk variants increased UMOD expression in vitro and in vivo. Uromodulin overexpression in transgenic mice led to salt-sensitive hypertension and to the presence of age-dependent renal lesions similar to those observed in elderly individuals homozygous for UMOD promoter risk variants. The link between uromodulin and hypertension is due to activation of the renal sodium cotransporter NKCC2. We demonstrated the relevance of this mechanism in humans by showing that pharmacological inhibition of NKCC2 was more effective in lowering blood pressure in hypertensive patients who are homozygous for UMOD promoter risk variants than in other hypertensive patients. Our findings link genetic susceptibility to hypertension and CKD to the level of uromodulin expression and uromodulin's effect on salt reabsorption in the kidney. These findings point to uromodulin as a therapeutic target for lowering blood pressure and preserving renal function.

PMID:
24185693
PMCID:
PMC3856354
DOI:
10.1038/nm.3384
[Indexed for MEDLINE]
Free PMC Article

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