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Lab Chip. 2013 Dec 21;13(24):4740-4. doi: 10.1039/c3lc50945a.

Probing cellular heterogeneity in cytokine-secreting immune cells using droplet-based microfluidics.

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1
Institute for Molecules and Materials, Radboud University Nijmegen, Heyendaalseweg 135, 6525 AJ Nijmegen, The Netherlands. w.huck@science.ru.nl.

Abstract

Here, we present a platform to detect cytokine (IL-2, IFN-γ, TNF-α) secretion of single, activated T-cells in droplets over time. We use a novel droplet-based microfluidic approach to encapsulate cells in monodisperse agarose droplets together with functionalized cytokine-capture beads for subsequent binding and detection of secreted cytokines from single cells. This method allows high-throughput detection of cellular heterogeneity and maps subsets within cell populations with specific functions.

PMID:
24185478
DOI:
10.1039/c3lc50945a
[Indexed for MEDLINE]
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