Format

Send to

Choose Destination
Lab Chip. 2013 Dec 21;13(24):4864-9. doi: 10.1039/c3lc50905b.

DNA sequence analysis with droplet-based microfluidics.

Author information

1
University of California, San Francisco - Bioengineering and Therapeutic Sciences, San Francisco, California, USA.

Abstract

Droplet-based microfluidic techniques can form and process micrometer scale droplets at thousands per second. Each droplet can house an individual biochemical reaction, allowing millions of reactions to be performed in minutes with small amounts of total reagent. This versatile approach has been used for engineering enzymes, quantifying concentrations of DNA in solution, and screening protein crystallization conditions. Here, we use it to read the sequences of DNA molecules with a FRET-based assay. Using probes of different sequences, we interrogate a target DNA molecule for polymorphisms. With a larger probe set, additional polymorphisms can be interrogated as well as targets of arbitrary sequence.

PMID:
24185402
PMCID:
PMC4090915
DOI:
10.1039/c3lc50905b
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Royal Society of Chemistry Icon for PubMed Central
Loading ...
Support Center