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Nanomedicine. 2014 Apr;10(3):525-34. doi: 10.1016/j.nano.2013.10.005. Epub 2013 Oct 30.

Polyxylitol-based gene carrier improves the efficiency of gene transfer through enhanced endosomal osmolysis.

Author information

1
Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea.
2
Department of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, China.
3
National Institute of Animal Science, Rural Development Administration, Suwon, South Korea.
4
Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, South Korea. Electronic address: chocs@plaza.snu.ac.kr.
5
Department of Agricultural Biotechnology, Seoul National University, Seoul, South Korea; Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, South Korea. Electronic address: cyjcow@snu.ac.kr.

Abstract

Endosomal escape is one of the important processes for efficient non-viral gene delivery. In this study, we synthesized a novel non-viral vector called polyxylitol-based gene carrier (XGC) through a Miachael addition reaction between xylitol diacrylate as a crosslinking agent and low molecular weight polyethylenimine (PEI 1.2kDa). The small amount of xylitol integrated into XGC (3.9% w/w) contributed 50% of the osmotic pressure of XGC, and enhaned the osmolysis of endosome cooperatively with the proton sponge effect, thus improving endosomal escape. Furthermore, XGC showed higher transfection efficiency in vivo in muscle tissue than pDNA alone or PEI 25kDa. In conclusion, our results show that XGC enhanced transfection efficiency compared with PEI 25kDa, the golden standard non-viral gene carrier, by enhancing endosomal escape without increasing the number of transfected cells.

FROM THE CLINICAL EDITOR:

Enhanced gene delivery methods would greatly facilitate the development of gene therapies. These authors demonstrate that a polyxylitol-based gene carrier enhanced the transfection efficiency compared with the gold standard non-viral gene carrier, as a result of enhancing endosomal escape without increasing the number of transfected cells, warranting further studies of this method.

KEYWORDS:

Endosomal escape; Gene delivery; Osmolysis; Polyethylenimine; Proton sponge

PMID:
24184000
DOI:
10.1016/j.nano.2013.10.005
[Indexed for MEDLINE]

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