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J Am Soc Mass Spectrom. 2014 Jan;25(1):6-9. doi: 10.1007/s13361-013-0765-z. Epub 2013 Oct 29.

Neutron-encoded protein quantification by peptide carbamylation.

Author information

1
Department of Chemistry, University of Wisconsin, Madison, WI, 53706, USA.

Abstract

We describe a chemical tag for duplex proteome quantification using neutron encoding (NeuCode). The method utilizes the straightforward, efficient, and inexpensive carbamylation reaction. We demonstrate the utility of NeuCode carbamylation by accurately measuring quantitative ratios from tagged yeast lysates mixed in known ratios and by applying this method to quantify differential protein expression in mice fed a either control or high-fat diet.

PMID:
24178922
PMCID:
PMC3880647
DOI:
10.1007/s13361-013-0765-z
[Indexed for MEDLINE]
Free PMC Article

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