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Methods Mol Biol. 2014;1093:59-72. doi: 10.1007/978-1-62703-694-8_6.

Biochemical and mass spectrometric analysis of 3'-end methylation of piRNAs.

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1
Department of Chemistry and Biotechnology, Graduate School of Engineering, University of Tokyo, Tokyo, Japan.

Abstract

Piwi-interacting RNAs (piRNA) are fully modified by 2'-O-methylation at their 3'-termini. This terminal methylation is required to prevent 3'-nucleotide addition, which serves as a tag for destabilization. In this chapter, we describe biochemical and mass spectrometric analyses of 2'-O-methylation at 3'-termini of piRNAs.

PMID:
24178557
DOI:
10.1007/978-1-62703-694-8_6
[Indexed for MEDLINE]
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