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Nucleic Acids Res. 2014 Feb;42(3):1887-92. doi: 10.1093/nar/gkt1022. Epub 2013 Oct 30.

Stimulation of ribosomal frameshifting by RNA G-quadruplex structures.

Author information

1
Department of Molecular Genetics, Leiden Institute of Chemistry, Leiden University, PO Box 9502, Leiden, The Netherlands and Institut Curie, UMR 176-CNRS, Bât 110, Université Paris-Sud, 91405 Orsay, France.

Abstract

Guanine-rich sequences can fold into four-stranded structures of stacked guanine-tetrads, so-called G-quadruplexes (G4). These unique motifs have been extensively studied on the DNA level; however, exploration of the biological roles of G4s at the RNA level is just emerging. Here we show that G4 RNA when introduced within coding regions are capable of stimulating -1 ribosomal frameshifting (-1 FS) in vitro and in cultured cells. Systematic manipulation of the loop length between each G-tract revealed that the -1 FS efficiency positively correlates with G4 stability. Addition of a G4-stabilizing ligand, PhenDC3, resulted in higher -1 FS. Further, we demonstrated that the G4s can stimulate +1 FS and stop codon readthrough as well. These results suggest a potentially novel translational gene regulation mechanism mediated by G4 RNA.

PMID:
24178029
PMCID:
PMC3919603
DOI:
10.1093/nar/gkt1022
[Indexed for MEDLINE]
Free PMC Article

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