Format

Send to

Choose Destination
See comment in PubMed Commons below
Micron. 2014 Jan;56:37-43. doi: 10.1016/j.micron.2013.10.002. Epub 2013 Oct 16.

X-ray solution structure of the native neuronal porosome-synaptic vesicle complex: Implication in neurotransmitter release.

Author information

1
Wayne State University School of Medicine, Department of Biochemistry and Molecular Biology, Detroit, MI, USA.

Abstract

Nanoportals at the cell plasma membrane called porosomes, mediate secretion from cells. In neurons porosomes are 15 nm cup-shaped lipoprotein structure composed of nearly 40 proteins. The size and complexity of the porosome has precluded determination of its atomic structure. Here we report at nanometer resolution the native 3D structure of the neuronal porosome-synaptic vesicle complex within isolated nerve terminals using small-angle X-ray solution scattering. In addition to furthering our understanding of the porosome structure, results from the study suggests the molecular mechanism involved in neurotransmitter release at the nerve terminal.

KEYWORDS:

Electron microscopy; Neuronal porosome complex; Photon correlation spectroscopy; X-ray solution scattering

PMID:
24176623
DOI:
10.1016/j.micron.2013.10.002
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center