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Vaccine. 2013 Dec 17;32(1):111-8. doi: 10.1016/j.vaccine.2013.10.056. Epub 2013 Oct 29.

Different T cell memory in preadolescents after whole-cell or acellular pertussis vaccination.

Author information

1
Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), Brussels, Belgium.
2
Pediatric Department, Hôpital Erasme, Université Libre de Bruxelles (ULB), Brussels, Belgium.
3
Department of Pediatric Pulmonology, Cystic Fibrosis Clinic and Pediatric Infectious Diseases, Universitair Ziekenhuis Brussel (UZ Brussel), Brussels, Belgium.
4
Department of Infectious, Parasitic and Immune-mediated Diseases, Istituto Superiore di Sanità, Rome, Italy.
5
INSERM U 1019, Lille, France; CNRS, UMR8204, Lille, France; Université Lille Nord de France, Lille, France; Institut Pasteur de Lille, Center for Infection and Immunity of Lille, Lille, France.
6
Laboratory of Vaccinology and Mucosal Immunity, Université Libre de Bruxelles (ULB), Brussels, Belgium; Immunobiology Clinic, Hôpital Erasme, Université Libre de Bruxelles (ULB), Brussels, Belgium. Electronic address: fmascart@ulb.ac.be.

Abstract

To better understand vaccine-induced protection and its potential failure in light of recent whooping cough resurgence, we evaluated quantity as well as quality of memory T cell responses in B. pertussis-vaccinated preadolescent children. Using a technique based on flow cytometry to detect proliferation, cytokine production and phenotype of antigen-specific cells, we evaluated residual T cell memory in a cohort of preadolescents who received a whole-cell pertussis (wP; n=11) or an acellular pertussis vaccine (aP; n=13) during infancy, and with a median of 4 years elapsed from the last pertussis booster vaccine, which was aP for all children. We demonstrated that B. pertussis-specific memory T cells are detectable in the majority of preadolescent children several years after vaccination. CD4(+) and CD8(+) T cell proliferation in response to pertussis toxin and/or filamentous hemagglutinin was detected in 79% and 60% of the children respectively, and interferon-γ or tumor necrosis factor-α producing CD4(+) T cells were detected in 65% and 53% of the children respectively. Phenotyping of the responding cells showed that the majority of antigen-specific cells, whether defined by proliferation or cytokine production, were CD45RA(-)CCR7(-) effector memory T cells. Although the time since the last booster vaccine was significantly longer for wP-compared to aP-vaccinated children, their proliferation capacity in response to antigenic stimulation was comparable, and more children had a detectable cytokine response after wP- compared to aP-vaccination. This study supports at the immunological level recent epidemiological studies indicating that infant vaccination with wP induces longer lasting immunity than vaccination with aP-vaccines.

KEYWORDS:

Acellular pertussis vaccine; Bordetella pertussis; Bp; Children; FHA; Immune response; Memory T cell; PBMC; PT; SEB; Staphylococcus enterotoxin B; Whole-cell pertussis vaccine; aP; acellular pertussis; filamentous hemagglutinin; peripheral blood mononuclear cells; pertussis toxin; wP; whole-cell pertussis

PMID:
24176499
DOI:
10.1016/j.vaccine.2013.10.056
[Indexed for MEDLINE]
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