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Nat Methods. 2013 Nov;10(11):1063-7. doi: 10.1038/nmeth.2697.

The need for transparency and good practices in the qPCR literature.

Author information

1
Postgraduate Medical Institute, Anglia Ruskin University, Chelmsford, UK.

Abstract

Two surveys of over 1,700 publications whose authors use quantitative real-time PCR (qPCR) reveal a lack of transparent and comprehensive reporting of essential technical information. Reporting standards are significantly improved in publications that cite the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, although such publications are still vastly outnumbered by those that do not.

PMID:
24173381
DOI:
10.1038/nmeth.2697
[Indexed for MEDLINE]

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