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Mol Cells. 2013 Oct;36(4):355-61. doi: 10.1007/s10059-013-0164-0. Epub 2013 Oct 22.

Enzymatic synthesis of apigenin glucosides by glucosyltransferase (YjiC) from Bacillus licheniformis DSM 13.

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1
Department of Pharmaceutical Engineering, Institute of Biomolecule Reconstruction, Sun Moon University, Asan, 336-708, Korea.

Abstract

Apigenin, a member of the flavone subclass of flavonoids, has long been considered to have various biological activities. Its glucosides, in particular, have been reported to have higher water solubility, increased chemical stability, and enhanced biological activities. Here, the synthesis of apigenin glucosides by the in vitro glucosylation reaction was successfully performed using a UDP-glucosyltransferase YjiC, from Bacillus licheniformis DSM 13. The glucosylation has been confirmed at the phenolic groups of C-4' and C-7 positions ensuing apigenin 4'-O-glucoside, apigenin 7-O-glucoside and apigenin 4',7-O-diglucoside as the products leaving the C-5 position unglucosylated. The position of glucosylation and the chemical structures of glucosides were elucidated by liquid chromatography/mass spectroscopy and nuclear magnetic resonance spectroscopy. The parameters such as pH, UDP glucose concentration and time of incubation were also analyzed during this study.

PMID:
24170092
PMCID:
PMC3887987
DOI:
10.1007/s10059-013-0164-0
[Indexed for MEDLINE]
Free PMC Article

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