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Methods Mol Biol. 2014;1098:47-55. doi: 10.1007/978-1-62703-718-1_4.

Gaussia luciferase-based mycoplasma detection assay in mammalian cell culture.

Author information

1
Experimental Therapeutics and Molecular Imaging Laboratory, Department of Neurology, Neuroscience Center, Massachusetts General Hospital, Boston, MA, USA.

Abstract

Mycoplasma contamination in mammalian cell culture is a common problem with serious consequences on experimental data, and yet many laboratories fail to perform regular testing. In this chapter, we describe a simple and sensitive mycoplasma detection assay based on the bioluminescent properties of the Gaussia luciferase reporter.

PMID:
24166367
DOI:
10.1007/978-1-62703-718-1_4
[Indexed for MEDLINE]

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