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AIDS Res Hum Retroviruses. 2014 Jan;30(1):25-8. doi: 10.1089/AID.2013.0239. Epub 2013 Nov 22.

Short communication: A low-cost method for analyzing nevirapine levels in hair as a marker of adherence in resource-limited settings.

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1
1 Division of HIV/AIDS, Department of Medicine, University of California , San Francisco, San Francisco, California.

Abstract

The measurement of antiretroviral concentrations in hair is emerging as an important technology to objectively quantify adherence to combination antiretroviral therapy. Hair levels of antiretrovirals are the strongest independent predictor of virologic success in large prospective cohorts of HIV-infected patients and surpass self-report in predicting outcomes. Hair is easy to collect and store, but validated methods to analyze antiretroviral levels in hair using liquid chromatography tandem mass spectrometry (LC-MS/MS) are expensive. We report here on the development of a thin-layer chromatography (TLC) assay for the semiquantitative analysis of nevirapine in hair. TLC assay results from 11 samples were consistent with results using LC-MS/MS [Spearman correlation coefficient 0.99 (95% CI 0.95-0.996)]. This simple, low-cost method of analyzing nevirapine concentrations in hair may provide a novel monitoring tool for antiretroviral adherence in resource-limited settings and merits further study in clinical settings.

PMID:
24164410
PMCID:
PMC3887402
DOI:
10.1089/aid.2013.0239
[Indexed for MEDLINE]
Free PMC Article
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