Signal-to-noise ratio estimation on SEM images using cubic spline interpolation with Savitzky-Golay smoothing

J Microsc. 2014 Jan;253(1):1-11. doi: 10.1111/jmi.12089. Epub 2013 Oct 24.

Abstract

A new technique based on cubic spline interpolation with Savitzky-Golay noise reduction filtering is designed to estimate signal-to-noise ratio of scanning electron microscopy (SEM) images. This approach is found to present better result when compared with two existing techniques: nearest neighbourhood and first-order interpolation. When applied to evaluate the quality of SEM images, noise can be eliminated efficiently with optimal choice of scan rate from real-time SEM images, without generating corruption or increasing scanning time.

Keywords: Additive white Gaussian noise; electron microscope; signal-to-noise ratio.

MeSH terms

  • Animals
  • Culicidae / ultrastructure
  • Extremities / anatomy & histology
  • Image Processing, Computer-Assisted / methods*
  • Microscopy, Electron, Scanning / methods*
  • Signal-To-Noise Ratio*