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Analyst. 2013 Nov 12;138(24):7294-8. doi: 10.1039/c3an01540h.

Single-channel electrophysiology of cell-free expressed ion channels by direct incorporation in lipid bilayers.

Author information

1
Electronics and Computer Science, University of Southampton, Southampton, SO17 1BJ, UK. mdp@ecs.soton.ac.uk.

Abstract

Single-channel electrophysiology with lipid bilayer systems requires ion channel expression, purification from cell culture, and reconstitution in proteoliposomes for delivery to a planar bilayer. Here we demonstrate that single-channel current measurements of the potassium channels KcsA and hERGS5-S6 can be obtained by direct insertion in interdroplet lipid bilayers from microliters of a cell-free expression medium.

PMID:
24162163
DOI:
10.1039/c3an01540h
[Indexed for MEDLINE]

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