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Lancet. 2014 Jan 18;383(9913):238-44. doi: 10.1016/S0140-6736(13)62033-4. Epub 2013 Oct 23.

The first tissue-engineered airway transplantation: 5-year follow-up results.

Author information

1
Department of General Thoracic and Regenerative Surgery, University Hospital Careggi, Florence, Italy.
2
Advanced Center for Translational Regenerative Medicine, Division of Ear, Nose, and Throat, Karolinska Institutet, Huddinge, Stockholm, Sweden.
3
Division of Pathological Anatomy, Department of Critical Care Medicine and Surgery, University of Florence, Florence, Italy.
4
Department of Experimental and Clinical Medicine, University of Florence, Florence, Italy.
5
Department of Respiratory Medicine, Hospital de la Santa Creu i Sant Pau, Institut d'Investigació Biomèdica Sant Pau, Universitat Autonoma de Barcelona, Barcelona, Spain.
6
Department of Biomedicine, University Hospital Careggi, Florence, Italy.
7
Advanced Center for Translational Regenerative Medicine, Division of Ear, Nose, and Throat, Karolinska Institutet, Huddinge, Stockholm, Sweden. Electronic address: paolo.macchiarini@ki.se.

Abstract

BACKGROUND:

In 2008, the first transplantation of a tissue-engineered trachea in a human being was done to replace an end-staged left main bronchus with malacia in a 30-year-old woman. We report 5 year follow-up results.

METHODS:

The patient was followed up approximately every 3 months with multidetector CT scan and bronchoscopic assessment. We obtained mucosal biopsy samples every 6 months for histological, immunohistochemical, and electron microscopy assessment. We also assessed quality of life, respiratory function, cough reflex test, and production and specificity of recipient antibodies against donor human leucocyte antigen.

FINDINGS:

By 12 months after transplantation, a progressive cicatricial stenosis had developed in the native trachea close to the tissue-engineered trachea anastomosis, which needed repeated endoluminal stenting. However, the tissue-engineered trachea itself remained open over its entire length, well vascularised, completely re-cellularised with respiratory epithelium, and had normal ciliary function and mucus clearance. Lung function and cough reflex were normal. No stem-cell-related teratoma formed and no anti-donor antibodies developed. Aside from intermittent bronchoscopic interventions, the patient had a normal social and working life.

INTERPRETATION:

These clinical results provide evidence that a tissue-engineering strategy including decellularisation of a human trachea, autologous epithelial and stem-cell culture and differentiation, and cell-scaffold seeding with a bioreactor is safe and promising.

FUNDING:

European Commission, Knut and Alice Wallenberg Foundation, Swedish Research Council, ALF Medicine.

PMID:
24161821
DOI:
10.1016/S0140-6736(13)62033-4
[Indexed for MEDLINE]

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