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J Virol Methods. 2014 Jan;195:247-9. doi: 10.1016/j.jviromet.2013.10.019. Epub 2013 Oct 24.

Comparison of a commercial dengue IgM capture ELISA with dengue antigen focus reduction microneutralization test and the Centers for Disease Control dengue IgM capture-ELISA.

Author information

1
University of Utah, ARUP Institute for Clinical and Experimental Pathology, Salt Lake City, UT, United States.

Abstract

Dengue (DENV) infection is caused by an arbovirus that is a member of the family Flaviviridae, genus Flavivirus. The diagnosis of acute dengue infection using clinical signs and symptoms can be difficult since the manifestations cannot be readily differentiated from other infections. Therefore the diagnosis of acute dengue infection depends upon laboratory assays. Dengue virus ELISAs have been designed for the detection of IgM and IgG antibodies in addition to nonstructural 1 (NS1) antigens. The InBios IgM Dengue ELISA was compared to the Antigen Focus Reduction Microneutralization Test (FRμNT90) and Centers for Disease Control Dengue IgM Capture-ELISA (CDC MAC-ELISA). The calculated sensitivity, specificity and agreement of the InBios ELISA compared to FRμNT90 and CDC MAC-ELISA was 88.7% (C.I. 81.4-93.7), 93.1% (C.I. 89.1-95.8) and 91.5% (C.I. 86.3-95.0), respectively. In summary the InBios IgM Dengue ELISA sensitivity and specificity is comparable to other commercially available IgM Capture-ELISAs.

KEYWORDS:

Antibodies; Arbovirus; Dengue; ELISA; Flavivirus; Microneutralization

PMID:
24161816
DOI:
10.1016/j.jviromet.2013.10.019
[Indexed for MEDLINE]

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