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Acta Histochem. 2014 Mar;116(2):407-14. doi: 10.1016/j.acthis.2013.09.004. Epub 2013 Oct 22.

Actin cytoskeleton reorganization correlates with polarization of secretory vesicle and cell morphology in the degranulation of mast cell subtypes in human colon tissues.

Author information

1
Laboratory of Analytical Cytology, Shantou University Medical College, Shantou 515041, People's Republic of China. Electronic address: lin.juelong@qq.com.
2
Laboratory of Analytical Cytology, Shantou University Medical College, Shantou 515041, People's Republic of China.
3
First Affiliated Hospital, Shantou University Medical College, Shantou 515041, People's Republic of China.
4
Institute of Oncologic Pathology, Shantou University Medical College, Shantou 515041, People's Republic of China.
5
Department of Biochemistry and Molecular Biology, Shantou University Medical College, Shantou 515041, People's Republic of China.

Abstract

Mast cells play a central role in the intestinal immune response. To investigate the relationship between degranulation, cell polarization and the reorganization of actin cytoskeleton of mast cells, we used fluorescence or gold labeling methods to identify different mast cell subtypes in human colon. The reorganization of filamentous actin was visualized and then the polarization of secretory vesicles, as well as cell surfaces, was analyzed by fluorescence microscopy and electron microscopy. Our results first showed a diversity of filamentous actin assembly or disassembly within the contacting cell membrane of different mast cell subtypes. The polarization and degranulation of secretory vesicles was not only accompanied with the assembly and disassembly of filamentous actin at the cell periphery, but also with changes of cell surface polarization. Our study provides an insight into the local membranous structures and suggested correlations of cytoskeleton arrangement with the polarization of secretory vesicles and cell surface configuration during mast cell degranulation.

KEYWORDS:

Filamentous actin reorganization; Mast cell subtypes; Secretory vesicle

PMID:
24161690
DOI:
10.1016/j.acthis.2013.09.004
[Indexed for MEDLINE]
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