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Insect Biochem Mol Biol. 2013 Dec;43(12):1189-200. doi: 10.1016/j.ibmb.2013.10.003. Epub 2013 Oct 23.

Early changes in the pupal transcriptome of the flesh fly Sarcophagha crassipalpis to parasitization by the ectoparasitic wasp, Nasonia vitripennis.

Author information

1
Laboratory of Zoophysiology, Ghent University, Krijgslaan 281 S2, B-9000 Ghent, Belgium. Electronic address: Ellen.Danneels@UGent.be.

Abstract

We investigated changes in the pupal transcriptome of the flesh fly Sarcophaga crassipalpis, 3 and 25 h after parasitization by the ectoparasitoid wasp, Nasonia vitripennis. These time points are prior to hatching of the wasp eggs, thus the results document host responses to venom injection, rather than feeding by the wasp larvae. Only a single gene appeared to be differentially expressed 3 h after parasitization. However, by 25 h, 128 genes were differentially expressed and expression patterns of a subsample of these genes were verified using RT-qPCR. Among the responsive genes were clusters of genes that altered the fly's metabolism, development, induced immune responses, elicited detoxification responses, and promoted programmed cell death. Envenomation thus clearly alters the metabolic landscape and developmental fate of the fly host prior to subsequent penetration of the pupal cuticle by the wasp larva. Overall, this study provides new insights into the specific action of ectoparasitoid venoms.

KEYWORDS:

Ectoparasitoid; Microarray; Nasonia vitripennis; Sarcophaga crassipalpis; Venom

PMID:
24161520
DOI:
10.1016/j.ibmb.2013.10.003
[Indexed for MEDLINE]

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