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Braz J Microbiol. 2013 May 31;44(1):287-9. doi: 10.1590/S1517-83822013000100042. eCollection 2013.

Measuring of Mycobacterium tuberculosis growth. A correlation of the optical measurements with colony forming units.

Author information

1
Laboratorio de Biología Molecular, Centro de Investigación Biomédica del Noreste, Instituto Mexicano del Seguro Social, Monterrey, NL, México. ; Posgrado en Microbiología, Facultad de Ciencias Biológicas, Universidad Autónoma de Nuevo León, San Nicolás de los Garza, NL, México.

Abstract

The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD600) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD600 units.

KEYWORDS:

McFarland; Mycobacterium tuberculosis; bacterial growth; colony forming units; optical density

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