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Methods Mol Biol. 2014;1087:29-36. doi: 10.1007/978-1-62703-670-2_3.

The fate of HIV-1 capsid: a biochemical assay for HIV-1 uncoating.

Author information

1
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY, USA.

Abstract

The uncoating process of HIV-1 is a poorly understood process, so the development of a reliable assay to study uncoating is critical for moving the field forward. Here we describe an uncoating assay that currently represents the state-of-the-art biochemical procedure for monitoring uncoating and core stability during infection. This assay is based on the biochemical separation of soluble capsid protein from particulate capsid cores and provides information about the fate of the capsid during infection.

PMID:
24158811
PMCID:
PMC4317566
DOI:
10.1007/978-1-62703-670-2_3
[Indexed for MEDLINE]
Free PMC Article

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