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Antivir Ther. 2014;19(1):89-95. doi: 10.3851/IMP2694. Epub 2013 Oct 24.

Sodium tanshinone IIA sulfonate inhibits porcine reproductive and respiratory syndrome virus via suppressing N gene expression and blocking virus-induced apoptosis.

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1
College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, PR China.

Abstract

BACKGROUND:

Porcine reproductive and respiratory syndrome virus (PRRSV) causes significant economic loss in the swine industry. Currently, there is no effective way to prevent PRRSV infection. Sodium tanshinone IIA sulfonate (STS), a natural compound derived from Salvia miltiorrhiza, was shown to possess anti-PRRSV activity, but the underlying mechanisms remain unclear. The objective of this study was to investigate the effect of STS on PRRSV-induced cell apoptosis and PRRSV N protein expression pattern.

METHODS:

Relative quantification real-time PCR was used to evaluate the inhibition of STS on N gene expression. Simultaneously indirect immunofluorescence assay (IFA) and western blot were used to assess the effect on N protein expression. Apoptosis was analysed using fluorescence microscope with an annexin V-EGFP kit. The effect of STS on caspase-3 cleaving was assessed by western blot.

RESULTS:

Our results showed that STS could inhibit viral N gene expression at both the messenger RNA stage and at the protein level in PRRSV-infected cells in a dose-dependent manner. In addition, STS could also rescue PRRSV-induced apoptosis.

CONCLUSIONS:

Our data suggest that STS may serve as a base compound for developing more effective drugs against PRRSV infection.

PMID:
24158620
DOI:
10.3851/IMP2694
[Indexed for MEDLINE]
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