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Genome Announc. 2013 Oct 24;1(5). pii: e00901-13. doi: 10.1128/genomeA.00901-13.

Complete Genome Sequence of a UL96 Mutant Cytomegalovirus Towne-BAC (Bacterial Artificial Chromosome) Isolate Passaged in Fibroblasts To Allow Accumulation of Compensatory Mutations.

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1
Department of Microbiology, University of Mississippi Medical Center, Jackson, Mississippi, USA.

Abstract

Here, we present the complete genome sequence of a cytomegalovirus Towne-BAC (bacterial artificial chromosome) isolate that we first genetically engineered to mutate the UL96 gene and then serially passaged in human fibroblasts to allow for the accumulation of compensatory mutations. A total of 17 single-base substitutions were discovered in the passaged genome compared to the reference sequence (KF493877).

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