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Nat Commun. 2013;4:2672. doi: 10.1038/ncomms3672.

Semiconductor-based DNA sequencing of histone modification states.

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1
Broad Institute of MIT and Harvard, Cambridge, Massachusetts 02142, USA.

Abstract

The recent development of a semiconductor-based, non-optical DNA sequencing technology promises scalable, low-cost and rapid sequence data production. The technology has previously been applied mainly to genomic sequencing and targeted re-sequencing. Here we demonstrate the utility of Ion Torrent semiconductor-based sequencing for sensitive, efficient and rapid chromatin immunoprecipitation followed by sequencing (ChIP-seq) through the application of sample preparation methods that are optimized for ChIP-seq on the Ion Torrent platform. We leverage this method for epigenetic profiling of tumour tissues.

PMID:
24157732
PMCID:
PMC3917140
DOI:
10.1038/ncomms3672
[Indexed for MEDLINE]
Free PMC Article
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