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Biomed Opt Express. 2013 Sep 13;4(10):2150-65. doi: 10.1364/BOE.4.002150. eCollection 2013.

Extracting three-dimensional orientation and tractography of myofibers using optical coherence tomography.

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Department of Electrical Engineering, Columbia University, 500 West 120th Street, New York, New York, USA.


Abnormal changes in orientation of myofibers are associated with various cardiac diseases such as arrhythmia, irregular contraction, and cardiomyopathy. To extract fiber information, we present a method of quantifying fiber orientation and reconstructing three-dimensional tractography of myofibers using optical coherence tomography (OCT). A gradient based algorithm was developed to quantify fiber orientation in three dimensions and particle filtering technique was employed to track myofibers. Prior to image processing, three-dimensional image data set were acquired from all cardiac chambers and ventricular septum of swine hearts using OCT system without optical clearing. The algorithm was validated through rotation test and comparison with manual measurements. The experimental results demonstrate that we are able to visualize three-dimensional fiber tractography in myocardium tissues.


(100.0100) Image processing; (110.4500) Optical coherence tomography; (170.6935) Tissue characterization

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