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Spectrochim Acta A Mol Biomol Spectrosc. 2014 Jan 24;118:806-10. doi: 10.1016/j.saa.2013.09.084. Epub 2013 Oct 8.

Detection of DNA damage based on metal-mediated molecular beacon and DNA strands displacement reaction.

Author information

1
Key Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, Jiangsu Province, PR China.

Abstract

DNA hairpin structure probes are usually designed by forming intra-molecular duplex based on Watson-Crick hydrogen bonds. In this paper, a molecular beacon based on silver ions-mediated cytosine-Ag(+)-cytosine base pairs was used to detect DNA. The inherent characteristic of the metal ligation facilitated the design of functional probe and the adjustment of its binding strength compared to traditional DNA hairpin structure probes, which make it be used to detect DNA in a simple, rapid and easy way with the help of DNA strands displacement reaction. The method was sensitive and also possesses the good specificity to differentiate the single base mismatched DNA from the complementary DNA. It was also successfully applied to study the damage effect of classic genotoxicity chemicals such as styrene oxide and sodium arsenite on DNA, which was significant in food science, environmental science and pharmaceutical science.

KEYWORDS:

DNA detection; DNA strands displacement reaction; Fluorescence; Silver ions-mediated molecular beacon

PMID:
24152865
DOI:
10.1016/j.saa.2013.09.084
[Indexed for MEDLINE]

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