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Small GTPases. 2013 Oct-Dec;4(4):231-5. doi: 10.4161/sgtp.26849. Epub 2013 Oct 22.

The CASZ1/Egfl7 transcriptional pathway is required for RhoA expression in vascular endothelial cells.

Author information

1
McAllister Heart Institute; University of North Carolina at Chapel Hill; Chapel Hill, NC USA; Department of Genetics; University of North Carolina at Chapel Hill; Chapel Hill, NC USA.
2
McAllister Heart Institute; University of North Carolina at Chapel Hill; Chapel Hill, NC USA; Departments of Pathology and Laboratory Medicine; University of North Carolina at Chapel Hill; Chapel Hill, NC USA.
3
McAllister Heart Institute; University of North Carolina at Chapel Hill; Chapel Hill, NC USA; Department of Biology; University of North Carolina at Chapel Hill; Chapel Hill, NC USA; Department of Genetics; University of North Carolina at Chapel Hill; Chapel Hill, NC USA.

Abstract

Vertebrate development depends on the formation of a closed circulatory loop consisting of intricate networks of veins, arteries, and lymphatic vessels. The coordinated participation of multiple molecules including growth factors, transcription factors, extracellular matrix proteins, and regulators of signaling such as small GTPases is essential for eliciting the desired cellular behaviors associated with vascular assembly and morphogenesis. We have recently demonstrated that a novel transcriptional pathway involving activation of the Epidermal Growth Factor-like Domain 7 (Egfl7) gene by the transcription factor CASTOR (CASZ1) is required for blood vessel assembly and lumen morphogenesis. Furthermore, this transcriptional network promotes RhoA expression and subsequent GTPase activity linking transcriptional regulation of endothelial gene expression to direct physiological outputs associated with Rho GTPase signaling, i.e., cell adhesion and cytoskeletal dynamics. Here we will discuss our studies with respect to our current understanding of the mechanisms underlying regulation of RhoA transcription, protein synthesis, and activity.

KEYWORDS:

CASZ1; Egfl7; RhoA; blood vessel development; transcriptional regulation

PMID:
24150064
PMCID:
PMC4011818
DOI:
10.4161/sgtp.26849
[Indexed for MEDLINE]
Free PMC Article

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