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Lab Chip. 2013 Dec 7;13(23):4563-72. doi: 10.1039/c3lc50736j.

Ultrahigh-throughput sorting of microfluidic drops with flow cytometry.

Author information

1
UC Berkeley-UCSF Graduate Program in Bioengineering, University of California, San Francisco, San Francisco, California, USA.

Abstract

The detection and sorting of aqueous drops is central to microfluidic workflows for high-throughput biology applications, including directed evolution, digital PCR, and antibody screening. However, high-throughput detection and sorting of drops require optical systems and microfluidic components that are complex, difficult to build, and often yield inadequate sensitivity and throughput. Here, we demonstrate a general method to harness flow cytometry, with its unmatched speed and sensitivity, for droplet-based microfluidic sorting.

PMID:
24146020
DOI:
10.1039/c3lc50736j
[Indexed for MEDLINE]

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