Format

Send to

Choose Destination
Nat Mater. 2013 Dec;12(12):1154-62. doi: 10.1038/nmat3777. Epub 2013 Oct 20.

Biophysical regulation of epigenetic state and cell reprogramming.

Author information

1
1] UC Berkeley & UCSF Joint Graduate Program in Bioengineering, Berkeley/San Francisco, California 94720/94143, USA [2] Department of Bioengineering, University of California, Berkeley, B108A Stanley Hall, Berkeley, California 94720-1762, USA.

Abstract

Biochemical factors can help reprogram somatic cells into pluripotent stem cells, yet the role of biophysical factors during reprogramming is unknown. Here, we show that biophysical cues, in the form of parallel microgrooves on the surface of cell-adhesive substrates, can replace the effects of small-molecule epigenetic modifiers and significantly improve reprogramming efficiency. The mechanism relies on the mechanomodulation of the cells' epigenetic state. Specifically, decreased histone deacetylase activity and upregulation of the expression of WD repeat domain 5 (WDR5)--a subunit of H3 methyltranferase--by microgrooved surfaces lead to increased histone H3 acetylation and methylation. We also show that microtopography promotes a mesenchymal-to-epithelial transition in adult fibroblasts. Nanofibrous scaffolds with aligned fibre orientation produce effects similar to those produced by microgrooves, suggesting that changes in cell morphology may be responsible for modulation of the epigenetic state. These findings have important implications in cell biology and in the optimization of biomaterials for cell-engineering applications.

Comment in

PMID:
24141451
DOI:
10.1038/nmat3777
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Nature Publishing Group
Loading ...
Support Center