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J Microbiol Methods. 2013 Dec;95(3):366-7. doi: 10.1016/j.mimet.2013.10.005. Epub 2013 Oct 16.

Interference of ferric ions with ferrous iron quantification using the ferrozine assay.

Author information

1
Department of Microbiology, University of Tennessee, Knoxville, TN 37996, USA; Center for Environmental Biotechnology, University of Tennessee, Knoxville, TN 37996, USA.

Abstract

The ferrozine assay is a widely used colorimetric method for determining soluble iron concentrations. We provide evidence for a heretofore unrecognized interference of ferric ions (Fe(3+)) on ferrous iron (Fe(2+)) measurements performed in the dark. Fe(3+) concentrations affected the absorbance measurements, which linearly increased with incubation time.

KEYWORDS:

Ferric; Ferrous; Ferrozine; Interference

PMID:
24140574
DOI:
10.1016/j.mimet.2013.10.005
[Indexed for MEDLINE]

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